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flag ikk2dn  (Addgene inc)


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    Structured Review

    Addgene inc flag ikk2dn
    Flag Ikk2dn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+flag+ikk%CE%B2+km/pCR-Flag-IKKbeta-KM+(Plasmid+%2315466)/pm38470493-238-0-1
    Average 90 stars, based on 9 article reviews
    flag ikk2dn - by Bioz Stars, 2026-09
    90/100 stars

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    Mutagenesis:

    Article Title: Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe
    Article Snippet: .. Flag-tagged IKKβ and its mutant were amplified from pCR-Flag-IKKβ (Addgene plasmid #15465) or pCR-Flag-IKKβ-KM (Addgene plasmid #15466) plasmids and inserted into pMRX-IB vector. .. The following plasmids were generated: pCW DNaseIIα-sfGFP-T2A-mCherry, pCW ASAH-sfGFP-T2A-mCherry, pCW LAMP1-sfGFP-T2A-mCherry, pMRX-IP TFEB-sfGFP, pMRX-IB CDK1-HA, pMRX-IB CDK4-HA, pMRX-IB CDK5-HA, pMRX-IB Flag-IKKβ, and pMRX-IB Flag-IKKβ (K44A). pCMV-VSVG (Addgene plasmid #8454) and psPAX2 (Addgene plasmid #12260) were used for lentivirus production. pCMV-VSVG and Gag were used for retrovirus production.

    Amplification:

    Article Title: Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe
    Article Snippet: .. Flag-tagged IKKβ and its mutant were amplified from pCR-Flag-IKKβ (Addgene plasmid #15465) or pCR-Flag-IKKβ-KM (Addgene plasmid #15466) plasmids and inserted into pMRX-IB vector. .. The following plasmids were generated: pCW DNaseIIα-sfGFP-T2A-mCherry, pCW ASAH-sfGFP-T2A-mCherry, pCW LAMP1-sfGFP-T2A-mCherry, pMRX-IP TFEB-sfGFP, pMRX-IB CDK1-HA, pMRX-IB CDK4-HA, pMRX-IB CDK5-HA, pMRX-IB Flag-IKKβ, and pMRX-IB Flag-IKKβ (K44A). pCMV-VSVG (Addgene plasmid #8454) and psPAX2 (Addgene plasmid #12260) were used for lentivirus production. pCMV-VSVG and Gag were used for retrovirus production.

    Plasmid Preparation:

    Article Title: Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe
    Article Snippet: .. Flag-tagged IKKβ and its mutant were amplified from pCR-Flag-IKKβ (Addgene plasmid #15465) or pCR-Flag-IKKβ-KM (Addgene plasmid #15466) plasmids and inserted into pMRX-IB vector. .. The following plasmids were generated: pCW DNaseIIα-sfGFP-T2A-mCherry, pCW ASAH-sfGFP-T2A-mCherry, pCW LAMP1-sfGFP-T2A-mCherry, pMRX-IP TFEB-sfGFP, pMRX-IB CDK1-HA, pMRX-IB CDK4-HA, pMRX-IB CDK5-HA, pMRX-IB Flag-IKKβ, and pMRX-IB Flag-IKKβ (K44A). pCMV-VSVG (Addgene plasmid #8454) and psPAX2 (Addgene plasmid #12260) were used for lentivirus production. pCMV-VSVG and Gag were used for retrovirus production.



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